The influence of simulated microgravity on the formation of storage polysaccharides of potato minitubers

1Shnyukova, EI, 2Nedukha, ОМ
1M.G. Kholodny Institute of Botany of the National Academy of Sciences of Ukraine, Kyiv
2M.G. Kholodny Institute of Botany of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
Kosm. nauka tehnol. 2004, 10 ;(5-6):229-232
https://doi.org/10.15407/knit2004.05.229
Publication Language: Ukrainian
Abstract: 
A simulated microgravity influenced essentially the metabolism of storage polysaccharides of the potato minitubers. An increase of synthesis and accumulation of starch and an enhancement of phosphorylase activity were observed in the minitubers during a long-term clinorotation. At the same time the mono- and disaccha-rides content decreased, which pointed to the intensification of their utilization for starch synthesis. The correlation between the enhancement of enzyme activity and the increase of starch content is revealed.
References: 
1. Bolotova V. C., Sakanyan E. I., Lesiovskaya E. E., Pastushenkov L. V. Spectrophotometric method for determination of polysaccharides in leaves of Tilia cordata Mill. Rastitel'nye resursy, 37 (3), 109—112 (2001) [in Russian].
 
2. Vul'fson P. A., Aleksahina N. V. Structure and function of phosphorylase. In: Struktura i funkcija fermentov, 60—82 (Nauka, Moscow, 1972) [in Russian].
 
3. Ermakov A. I., Arasimovich V. V., Yarosh N. P., et al. Methods of biochemical investigation of plants, Ed. by A. I. Ermakov, 3rd ed., 430 p. (Agropromizdat, Leningrad, 1987) [in Russian].
 
4. Rodionov V. S., Kholoptseva N. P. Determination of plant leaf phospholipids using two-dimensional chromatography in the thin layers of silica gel. Fiziol. biokhim. kult. rast., 6 (2), 67—87 (1974) [in Russian].
 
5. Gerbrandy S. J., Verleur J. D. Phosphorylase isoenzymes: localization and occurrence in different plant orgains in relation to starch metabolism. Phytochemistry, 10 (2), 261—266 (1971).
 
6. Kordyum E. Biology of plant cells in microgravity and under clinostating. Int. Rev. Cytol., 171, 1—78 (1997).
 
7. Kordyum E., Baranenko V., Nedukha O., Samoilov V. Development of potato minitubers in microgravity. Plant Cell Physiol., 38, 1111 — 1117 (1997).
 
8. Lowry O. H., Rosenbrough N. J., Farr A. L., Randall R. I. Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193 (1), 265—275 (1951).
 
9. Nedukha O. M., Schnyukova E. I., Leach J. E. High phosphorylase activity is corrected with increased potato minituber formation and starch content during extended clinorotation. Adv. Space Res., 31 (10), 2245—2251 (2003).