Calcium in gravitropism of the moss Pohlia nutans (Hedw.) Lindb. protonemata

1Khorkavtsiv, OYa., 1Demkiv, OT, 1Khorkavtsiv, Ya.D
1Institute of Ecology of the Carpathians of the National Academy of Sciences of Ukraine, L’viv, Ukraine
Kosm. nauka tehnol. 2002, 8 ;(1):089-095
Section: Space Life Sciences
Publication Language: Ukrainian
Protonemata of mosses both of Pohlia nutans grow by extension and division of single apical cells which are negatively gravitropic in darkness. The fluorescence of Ca2+ increased in the tip of apical cells from the first hours of gravitropism stimulation. Cytochemical investigations confirmed the existence of a well pronounced tip-to-base Ca2+-gradient, its formation being favoured by localization of calcium influx in the tip of the apical cell. Measurement of the cytochemical reaction intensity showed that the level of Ca2+-ATFase activity is low in apex and increases towards the base of the apical cell. The gravitropic protonemata and filaments which grew on the clinostat showed similar distributions of the Ca2+ and Ca2+-ATFase activity along the apical cell axis. Thus, these data demonstrate that growing apical cells of gravitropic protonemata have a high tip-to-base Ca2+ gradient, the Ca 2+ transport being afforded by Ca 2+-ATFase.
Keywords: apical cell, cytochemical investigations, protonemata
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