The influence of slow clinorotation on the expression of small heat shock proteins in Pisum sativum L.

1Talalaiev, OS
1M.G. Kholodny Institute of Botany of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
Kosm. nauka tehnol. 2007, 13 ;(2):053-056
Publication Language: Ukrainian
The stress gene expression in Pisum sativum L. seedlings exposed to altered gravity and temperature elevation is evaluated. We investigate message for two inducible forms of cytosolic small heat shock proteins (sHsp), sHsp 17.7 and sHsp 18.1. Both proteins are able to enhance the refolding of chemically denatured proteins in ATP-independent manner, i.e., they can function as molecular chaperones. The sHsp expression in pea seedling cells is studied by Western blotting. Applying the RT-PCR, we explore sHsp mRNA. Temperature elevation, as the positive control, significantly increased the PsHsp 17.7 and PsHsp 18.1 expression. The expression of the housekeeping protein, actin, was constant and comparable to unstressed controls for all of the treatments. We can conclude that gravitational perturbations incurred by clinorotation do not change the sHsp gene expression.
Keywords: molecular chaperones, proteins, refolding
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