Effects of clinorotation on wheat's resistance to viral infection

Heading: 
Space Life Sciences
1Mischenko, LT, 1Ostapchenko, LI, 1Filenko, OM
1Taras Shevchenko National University of Kyiv, Kyiv, Ukraine
Kosm. nauka tehnol. 2005, 11 ;(1-2):087-092
https://doi.org/10.15407/knit2005.01.087
Publication Language: Ukrainian
Abstract: 
The effects of vertical and horizontal clinorolation on the Apogee wheal's resistance lo viral infection a studied at a container velocity of rotation of 2 rpm and a platform velocity of rotation of І фін. Рог the first time, it became possible to oblain the Apogee wheat's yield of grain under simulated microgravily conditions for holh, nonin-fected and infected with the wheat streak mosaic virus (WSMV) wheat plants. Evidently, the process of plant adaptation to stress agents involves certain mechanisms, like PR-protection synthesis ot some, other. For the first lime, the character of viral infection dynamics with prolonged clinorolation is elucidated and the presence of the WSMV in wheal plants on the 18 day after inoculation is detected using Ihc procedure of polymerase chain reaction (PCR). Vertical clinorolation proved the least suitable experimental variant for viral reproduction. Increase in total nitrogen conienl .n spikes, which occurred in this variant, gives grounds to suggest a possibility of interferon-like protein synthesis in plants similar to that of interferon system in animals.
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References: 
1. Ermakov A. I. Methods of biochemical investigation of plants, 456 p. (Kolos, Leningrad, 1972) [in Russian].
2. Ladygina M. E., Babosha A. V. Physiological and biochemical nature of viral pathogenesis, stability and regulation of anti-infective activity. Fiziologija rastenij, 43 (5), 729—742 (1996) [in Russian].
3. Levinskikh M. A. Ontogeny, reproduction and metabolism of higher plants in space flight conditions: Extended abstract of Doctor’s thesis, 49 p. (In-t Mediko-biologicheskih problem RAN, Moscow, 2002) [in Russian].
4. Mischenko L. T. The effect of artificial gravity on grows processes and photosynthetic apparatus of Triticum aestivum L. infected by the wheat streak mosaic virus. Kosm. nauka tehnol., 8 (5-6), 66—70 (2002) [in Russian].
5. Mishchenko L. T., Kühne T., Mishchenko I. A., Boyko A. L. Infection process of wheat streak mosaic virus in clinostated Apogee wheat plants. Kosm. nauka tehnol., 9 (5-6), 211—215 (2003) [in Russian].
6. Roit A., Brostoff J., and Mail D. Immunology, 582 p. (Mir, Moscow, 2000) [in Russian].
7. Shcherbatenko I. S. Use of natural and induced resistance of plants to protect them from viral diseases. Mikrobiologichnyj zhurn., 60 (6), 56—65 (1998) [in Russian].
8. Shcherbatenko I. S. Plant resistance to viruses. Mikrobiologichnyj zhurn., 58 (2), 81—101 (1996) [in Ukrainian].
9. Chen J., Adams M. J. A universal PCR primer to deled members of the Potyviridae and its use to examine the taxonomic status of several members of the family. Arch. Virol., 146, 757—766 (2001).
https://doi.org/10.1007/s007050170144
10. Kordyum E. L., Shevchenko G. V. Role of cytoskeleton in plain cell gravisensitivity. J. Gravitational Physiology, 10 (1), 15—16 (2003).
11. Kuang A., Popova A., Xiao Yi., Musgrave M. E. Pollination and embryo development in Brassica rapa L. in microgravity Internal. J. Plant Sci., 161, 203—211 (2000).
https://doi.org/10.1086/314254
12. Mishchenko L. T. Dynamic of the WSMV reproduction in the Apogee wheat variety under simulated microgravity conditions. J. Grav. Physiol., 10 (1), 27—28 (2003).

13. Monje O., Bingham G. E., Carman J. G., et al. Canopy photosynthesis and transpiration in microgravity: gas exchange measurements aboard Mir. Adv. Space Res., 26 (2), 303—306 (2000).