Embryo development in Brassica rapa L. plants under clinorotation

1Popova, AF, 1Ivanenko, GF
1M.G. Kholodny Institute of Botany of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
Kosm. nauka tehnol. 2003, 9 ;(Supplement2):041-043
Publication Language: Ukrainian
Our results of comparative study of embryo formation in Brassica rapa L. plants under slow horizontal clinorotation and in laboratory control are presented. The research is executed on embryos of identified age, which was achieved by the marking of flowers after artificial pollination and by the use of temporal fixation of the plant material. Successive stages of embryo development up to their full differentiation (3–21 days after artificial pollination) are investigated. A significant similarity of peculiarities and rates of embryo development at early stages of embryogenesis is revealed in both variants. As a whole, the differentiation process of embryos in conditions of slow horizontal clinorotation and in the laboratory control proceeded identically. However, some cases of disturbance in the cotyledons and radicle development of embryos, generated under altered gravity, are noted. Disturbances revealed in embryo differentiation can be a reason of decrease of quantity and viability of seeds generated under changed gravity.
1. Levinskikh M. A., Sychev V. N., Derendiaeva T. A., et al. The influence of space flight factors on the growth and development of super dwarf wheat cultivated in greenhouse Svet. Aviakosm. Ekolog. Med., 33, 30—37 (1999) [in Russian].
2. Merkys A. J., Laurinavichius R. S. Complete cycle of individual development of Arabidopsis thaliana (L.) Heynh. plants on board the “Salyut-7” orbital station. Dokl. Academy of Sciences of the USSR, 271, 509 — 512 (1983) [in Russian].
3. Merkis F. I., Laurinavichyus R. S., Rupainene O. Yu., et al. Growth and development of plants under conditions imitating weightlessness. Dokl. Academy of Sciences of the USSR, 226, 978—981 (1976) [in Russian].
4. Bingham G. E., Sytchev V. N., Levinskikh M. A., Podolsky I. G. Final plant experiments on Mir provide second-generation wheat and seeds. Gravitat. and Space Biol. Bulletin, 13 (1), 48 (1999).
5. Eastmond P., Kolacna L., Rawsthorne S. Photosynthesis by developing embryos of oilseed rape (Brassica napus L.). J. Experim. Bot., 47 (304), 763—1769 (1996).
6. Kuang A., Popova A., Xiao Y., Musgrave M. Pollination and embryo development in Brassica rapa L. in microgravity. Internat. J. of Plant Sci., 161 (2), 203—211 (2000).
7. Musgrave M. E., Kuang A., Xiao Y., et al. Gravity-independence of seed-to-seed cycling. Planta, 210, 400—406 (2000).

8. Popova A., Musgrave M., Kuang A., and Xiao Yi. Reserve nutrient substance accumulation in Brassica rapa L. seeds in microgravity conditions (STS-87). J. Gravitat. Physiol., 9 (1), 237—238 (2002).