Modeling the pNARSLux transfer in the wheat rhizosphere under simulated microgravity

1Kovalchuk, MV, 1Negrutska, VV, 1Kovtunovych, GL, 1Lar, EV, 1Korniichuk, ОS, 1Rogutskyi, IS, 1Alpatov, AP, 1Kozyrovska, NA, 1Kordyum, VA
1Institute of Molecular Biology and Genetics of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
Kosm. nauka tehnol. 2003, 9 ;(Supplement2):010-014
Publication Language: English
Abstract: 
Model experiments on conjugal plasmid transfer between bacteria in the wheat rhizosphere under simulated microgravity (SMG) were performed to evaluate potential of natural gene flow and risk of genetic recombinations in microorganisms in space gardens under stressed conditions of space flight. Strains of bacteria Klebsiella oxytoca IMBG 26, Pseudomonas sp. 7, P. putida 9, Agrobacterium tumefaciens 9023, Escherichia coli S-17 were selected for matings in the wheat microcosm on a clinostate with horizontal mode of rotations within a ten-day period. Transfer of pNARSLux was not detected in both clinorotated and control variants in sterile conditions. The changed vector of gravity influenced both plant development, resulted in a shorter seedling heights, and in bacteria spread on the surface of wheat roots resulted in a less amount of cells. In both variants of experiments (normal gravity and SMG) positive impact of bacteria on wheat seedling development is displayed as compared with control noninoculated plants.
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