Effects of hypergravity stress on intensities of gaseous exchange, RNA and protein synthesis, thermoregulation, and survival of animals of different species

1Frol'kis, VV, 2Muradian, Kh.K, 1Timchenko, AN, 1Mozzhukhina, TG
1State Institution "Institute of Gerontology im.D.F.Chebotarova National Academy of Medical Sciences of Ukraine", Kyiv, Ukraine
2State Institution "D.F.Chebotarov Institute of Gerontology of the National Academy of Medical Sciences of Ukraine", Kyiv, Ukraine
Kosm. nauka tehnol. 1997, 3 ;(2):16-21
https://doi.org/10.15407/knit1997.03.016
Section: Space Life Sciences
Publication Language: Russian
Abstract: 
The effects of hypergravity (HG) stress on survival, gaseous exchange, thermoregulation, and synthesis of total RNA and protein were studied in adult male drosophilas, mice, and rats. Drosophilas had much higher HG tolerance (up to 20000 g) compared to mice and rats, for whom LD50 were 55—60g and 25g, correspondingly at a 10-minute exposure. In the groups of mice and rats, the gaseous exchange increased in the range of mild and sublethal HG, but it decreased in the lethal range of HG stress. In this range the body temperature, as well as RNA and protein synthesis in myocardium, frontal cortex, and hypothalamus also decreased; by contrast, synthesis processes increased in cerebrellum. In the post-stress period the gaseous exchange and body temperature recovered in about 1-2 hours.
Keywords: RNA and protein synthesis, space life sciences, space medicine
References: 
Clemens M. J. Translation of eukaryotic messenger RNA in cdl-free extracts. In: Hames B. D., Higgins S. J. (Eds.) Transcription and translation. A practical approach, 327—362 (Mir, Moscow, 1987) [in Russian].
Marzluff W. F., Huang R. C. C. Transcription of RNA in isolated nuclei. In: Hames B. D., Higgins S. J. (Eds.) Transcription and translation. A practical approach, 111 — 157 (Mir, Moscow, 1987) [in Russian].
Economos A. C., Miquel J., Ballard R. W., et  al. Effects of stimulated increased gravity on the rate of aging of rats. Implications for the rate of living theory of aging. Arch. Geront. and Geriatrics, 1, 349—363 (1983).
https://doi.org/10.1016/0167-4943(82)90035-8
Frolkis V. V., Muradian Kh. K., Timchenko A. N. Hypergravity stress: species- and age-peculiarities of oxygen consumption, thermoregulation, and survival. III European Congress of Gerontology: Abstracts (Amsterdam, 30 August—2 September, 1995), N 086.0078 (Amsterdam, 1995).
Galileo G. Discorsi e demonstrazioni matematiche intorno a due nuove science. 1638 cit. ex: Miquel, Souza (1991).
Hughes-Fulford M. Altered cell function in microgravity. Exp. Gerontol., 26 (2/3), 247—256 (1991).
https://doi.org/10.1016/0531-5565(91)90017-G
Le Bourg E., Lints F. A. Hypergravity and aging in Drosophila melanogaster. 2. Longevity. Gerontology, 35, 244—252 (1989).
https://doi.org/10.1159/000213033
Lee R. E., Bryant E. H., Baust J. G. Fecundity and longevity of housefly after spaceflight. Experientia, 41, 1191 — 1192 (1985).
https://doi.org/10.1007/BF01951723
Lints F. A., Bullens P., Le Bourg E. Hypergravity and longevity in Drosophila melanogaster. 7. New longevity data. Exp. Ge­rontol., 28, 611—615 (1993).
https://doi.org/10.1016/0531-5565(93)90050-N
Malacinski G. M., Neff A. W. The influence of gravity in the process of development of animal systems. Adv. Space Res., 4, 314—323 (1984).
https://doi.org/10.1016/0273-1177(84)90576-3
Markwell M. A., Haas S. H., Bieber L. L., Tolbert N. F. Modification of Lowry procedure to simplify protein determination in memb­rane and lipoprotein samples. Anal. Biochem., 85 (L), 206—210 (1978).
https://doi.org/10.1016/0003-2697(78)90586-9
Miquel J., Souza K. A. Gravity effects on reproduction, development, and aging. Advances in Space Biology and Medicine, 1, 71—97 (1991).
https://doi.org/10.1016/S1569-2574(08)60121-5
Nicogossian A. E., Yuntoon C. L., Pool S. L. Space Physiology and Medicine, 478 p. (Lea & Febiger, Philadelphia, 1989).
Pace N., Smith A. H. Gravity and metabolic scale effects in mammals. The Physiologist, 24, S37—40 (1981).
Singh N. P. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res., 175, 184—191 (1988).
https://doi.org/10.1016/0014-4827(88)90265-0
Wunder C. C. Gravitational aspects of growth of fruit fly larvae. Proc. Soc. for Exp. Biol. and Med., 89, 544 p. (1955).